BCCIP Antikörper (AA 167-300)

Produktnummer ABIN7871194

Der Kaninchen Polyklonal anti-BCCIP Antikörper (ABIN7871194) detektiert spezifisch BCCIP in WB, IHC, ELISA, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für BCCIP Antikörper (AA 167-300) (ABIN7871194)

Target: BCCIP (BRCA2 and CDKN1A Interacting Protein (BCCIP))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BCCIP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-BCCIP Antikörper

Bindungsspezifität: AA 167-300

Verwendungszweck: BCCIP Antibody / BRCA2 and CDKN1A-interacting protein

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human BCCIP recombinant protein (Position: K167-K300) was used as the immunogen for the BCCIP antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the BCCIP antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the BCCIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu BCCIP

Target: BCCIP (BRCA2 and CDKN1A Interacting Protein (BCCIP))

Andere Bezeichnung: BCCIP

Hintergrund: BCCIP antibody detects BRCA2 and CDKN1A-interacting protein, a nuclear protein involved in DNA repair, cell cycle regulation, and genome maintenance. Encoded by the BCCIP gene on chromosome 10q26.2, this protein interacts with BRCA2 and p21 (CDKN1A) to facilitate homologous recombination and checkpoint control. BCCIP functions as a cofactor in the BRCA2-RAD51 repair complex, promoting the repair of double-strand breaks and ensuring replication fork stability during DNA synthesis.

Structurally, BCCIP contains conserved coiled-coil domains and an acidic region that mediate protein-protein interactions with DNA repair and replication factors. It is expressed in proliferating cells and localizes primarily to the nucleus, particularly at sites of DNA damage. Through its association with p21, BCCIP also influences CDK activity and contributes to G1/S and G2/M checkpoint control.

The BCCIP antibody is widely used in DNA repair, oncology, and cell cycle research to study BRCA2-associated homologous recombination and genomic integrity. Western blot analysis identifies a 36 kilodalton band corresponding to BCCIP, while immunofluorescence shows nuclear punctate staining consistent with DNA damage foci. This antibody enables detection of repair complex dynamics and checkpoint activation in mammalian cells.

Dysregulation of BCCIP expression impairs DNA repair fidelity, leading to chromosomal instability, replication stress, and increased tumor susceptibility. Reduced BCCIP levels have been observed in glioblastoma and colorectal cancer, supporting its role as a tumor suppressor. The BCCIP antibody provides a reliable reagent for exploring DNA damage response and tumorigenic mechanisms. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, ensuring consistent results for genomic stability studies.

UniProt: Q9P287