ODF2 Antikörper (AA 11-829)

Produktnummer ABIN7870094

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ODF2 in WB, ELISA, IHC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für ODF2 Antikörper (AA 11-829) (ABIN7870094)

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ODF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-ODF2 Antikörper

Bindungsspezifität: AA 11-829

Verwendungszweck: ODF2 Antibody / Outer dense fiber protein 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human ODF2 recombinant protein (Position: R11-A829) was used as the immunogen for the ODF2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the ODF2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the ODF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu ODF2

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Andere Bezeichnung: ODF2

Hintergrund: ODF2 antibody detects Outer dense fiber protein 2, encoded by the ODF2 gene on chromosome 9q34.11. ODF2 antibody is used to study this coiled-coil structural protein, which is a key component of outer dense fibers in the sperm tail and the centrosomal appendages of somatic cells. ODF2 provides structural support to sperm flagella, ensuring integrity during motility, and contributes to centrosome function, microtubule anchoring, and ciliogenesis. Its dual role in reproductive biology and cell cycle regulation makes ODF2 a protein of wide research interest.

Structurally, ODF2 is a coiled-coil protein that assembles into filaments. In sperm, ODF2 filaments wrap around axonemal microtubules, forming outer dense fibers that stabilize the flagellum and protect it against mechanical stress. In somatic cells, ODF2 localizes to subdistal and distal appendages of centrioles, where it serves as a scaffold for proteins involved in ciliogenesis and microtubule anchoring. This dual localization underscores ODF2's evolutionary adaptation from reproductive structures to general centrosome biology.

Functionally, ODF2 plays a critical role in sperm motility and fertility. Knockout mouse models show that absence of ODF2 leads to abnormal sperm tails, defective motility, and infertility. In somatic cells, loss of ODF2 disrupts ciliogenesis, impairs microtubule organization, and causes defects in signaling pathways mediated by primary cilia. ODF2 is also required for centriole maturation and stability, influencing mitotic spindle organization and accurate chromosome segregation. Researchers use ODF2 antibody to study these processes in reproductive biology and cell cycle regulation.

Clinically, mutations or altered expression of ODF2 are associated with male infertility syndromes and ciliopathies, including primary ciliary dyskinesia. Dysregulation of ODF2 may also contribute to cancer through defective centrosome function, leading to chromosomal instability. Expression studies using ODF2 antibody demonstrate strong staining in testis and centrosome-associated structures in diverse tissues. NSJ Bioreagents offers ODF2 antibody to support investigations into sperm biology, centrosome function, and ciliary signaling.

UniProt: Q5BJF6

Pathways: M Phase