LSM14A Antikörper (AA 105-404)

Produktnummer ABIN7869977

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LSM14A in WB, ELISA, IF, IP, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für LSM14A Antikörper (AA 105-404) (ABIN7869977)

Target: LSM14A (LSM14A, SCD6 Homolog A (LSM14A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LSM14A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-LSM14A Antikörper

Bindungsspezifität: AA 105-404

Verwendungszweck: LSM14A Antibody / RAP55 / AlphaSNBP

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human LSM14A recombinant protein (Position: F105-R404) was used as the immunogen for the LSM14A antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the LSM14A antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the LSM14A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu LSM14A

Target: LSM14A (LSM14A, SCD6 Homolog A (LSM14A))

Andere Bezeichnung: LSM14A

Hintergrund: LSM14A antibody detects Sm-like protein LSM14A, a cytoplasmic RNA-binding protein that functions in mRNA storage, decay, and translational control. LSM14A is a core component of processing bodies (P-bodies), dynamic cytoplasmic granules involved in mRNA surveillance and post-transcriptional gene regulation. It plays a key role in mRNA decapping and interacts with proteins such as DDX6, EDC3, and PATL1 to form ribonucleoprotein complexes. The LSM14A antibody is essential for studying mRNA metabolism, stress response, and the regulation of gene expression at the post-transcriptional level.

LSM14A is encoded by the LSM14A gene located on human chromosome 19p13.2. The protein is approximately 46 kilodaltons and contains an Sm-like domain that mediates RNA binding and protein-protein interactions. It also includes a C-terminal FDF motif required for association with DDX6 helicase, a central regulator of mRNA decapping and translational repression. LSM14A localizes to discrete cytoplasmic foci corresponding to P-bodies, which dynamically assemble and disassemble in response to stress and cellular signaling. Through its activity, LSM14A influences mRNA stability and translation during differentiation and immune activation.

The LSM14A antibody reveals strong cytoplasmic punctate staining under conditions that promote P-body formation, such as serum starvation or oxidative stress. Western blot analysis typically detects a single band near 51 kilodaltons. Functional studies demonstrate that loss of LSM14A impairs mRNA decay, leading to prolonged expression of transiently expressed transcripts. Moreover, LSM14A participates in innate immune responses by regulating the stability of interferon-related mRNAs, linking RNA metabolism to antiviral defense mechanisms.

Recent research indicates that LSM14A interacts with other RNA granule components, including GW182 and AGO2, bridging mRNA silencing and decay pathways. It also contributes to stress granule dynamics and may influence autophagy signaling through mRNA control. Dysregulation of LSM14A expression has been observed in certain cancers and neurodegenerative diseases, highlighting its importance in cellular stress adaptation. NSJ Bioreagents provides a validated LSM14A antibody optimized for western blot, immunocytochemistry, and confocal microscopy applications. This reagent enables researchers to dissect mRNA storage and degradation pathways as well as stress-responsive post-transcriptional regulation.

UniProt: Q8ND56

Pathways: Activation of Innate immune Response, Ribonucleoprotein Complex Subunit Organization