GABBR2 Antikörper (AA 100-889)

Produktnummer ABIN7869882

Der Kaninchen Polyklonal anti-GABBR2 Antikörper wird verwendet zum Nachweis von GABBR2 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für GABBR2 Antikörper (AA 100-889) (ABIN7869882)

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABBR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-GABBR2 Antikörper

Bindungsspezifität: AA 100-889

Verwendungszweck: GABBR2 Antibody / GABA-B receptor 2

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human GABBR2 recombinant protein (Position: D100-Q889) was used as the immunogen for the GABBR2 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the GABBR2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the GABBR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu GABBR2

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Andere Bezeichnung: GABBR2

Hintergrund: GABBR2 antibody recognizes gamma-aminobutyric acid type B receptor subunit 2, a critical component of the GABA-B receptor complex that mediates inhibitory neurotransmission in the central nervous system. The GABA-B receptor is a G-protein-coupled receptor (GPCR) that functions as an obligate heterodimer composed of GABBR1 and GABBR2 subunits. While GABBR1 primarily binds the neurotransmitter GABA, GABBR2 is essential for G-protein coupling and signal transduction. Together, they regulate synaptic transmission, neuronal excitability, and plasticity by modulating calcium and potassium channels. The GABBR2 antibody is widely used in neuroscience research to study receptor distribution, intracellular trafficking, and synaptic physiology, as well as in models of epilepsy, addiction, and neurodegeneration.

GABBR2 is a transmembrane protein encoded by the GABBR2 gene, located on human chromosome 9q22.33. It contains a large extracellular Venus flytrap domain, a seven-transmembrane GPCR domain, and a long cytoplasmic tail involved in receptor assembly and signaling. The interaction of GABBR2 with GABBR1 is required for proper receptor localization at the cell surface. Dysregulation or mutations in GABBR2 have been associated with neurodevelopmental disorders, such as Rett-like syndrome and epileptic encephalopathy, emphasizing its importance in synaptic regulation. Experimental models have shown that altered GABA-B receptor function contributes to impaired inhibitory signaling and enhanced neuronal hyperexcitability.

The GABBR2 antibody is particularly valuable in immunohistochemistry for mapping GABA-B receptor expression across different brain regions, including the hippocampus, cortex, cerebellum, and spinal cord. Western blot analysis using this antibody often reveals bands around 100-110 kDa, corresponding to the mature receptor subunit. It is also useful in co-immunoprecipitation studies to confirm receptor dimerization with GABBR1 and interaction with downstream signaling molecules such as G-proteins and adenylate cyclases. Because of its high specificity and reproducibility, this antibody has become a standard reagent for characterizing the molecular and cellular mechanisms of inhibitory neurotransmission. NSJ Bioreagents provides a validated reagent that supports diverse applications including immunofluorescence and confocal microscopy to visualize receptor localization at synapses.

Research into GABBR2 continues to illuminate its roles beyond classical neurotransmission. Studies have linked GABA-B receptor signaling to cognitive flexibility, mood regulation, and drug dependence. Pharmacological modulation of this receptor has shown promise in treating anxiety, spasticity, and addiction disorders. The availability of a reliable GABBR2 antibody facilitates continued exploration into the receptor's signaling mechanisms, post-translational modifications, and potential therapeutic targets in neurological and psychiatric diseases.

UniProt: O75899

Pathways: cAMP Metabolic Process