MAML1 Antikörper (AA 1-998)

Produktnummer ABIN7869672

Der Kaninchen Polyklonal anti-MAML1 Antikörper wird verwendet zum Nachweis von MAML1 in Proben von Human. Er wurde validiert für ELISA, WB, FACS und IF.

Kurzübersicht für MAML1 Antikörper (AA 1-998) (ABIN7869672)

Target: MAML1 (Mastermind-Like 1 (MAML1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAML1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-MAML1 Antikörper

Bindungsspezifität: AA 1-998

Verwendungszweck: MAML1 Antibody / Mastermind-like protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human MAML1 recombinant protein (Position: M1-K998) was used as the immunogen for the MAML1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MAML1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MAML1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MAML1

Target: MAML1 (Mastermind-Like 1 (MAML1))

Andere Bezeichnung: MAML1

Hintergrund: MAML1 antibody detects Mastermind-like protein 1, a transcriptional coactivator that plays a key role in the Notch signaling pathway, which regulates cell fate determination, differentiation, and proliferation. Encoded by the MAML1 gene on chromosome 5q35.3, this nuclear protein acts as a core component of the Notch transcriptional activation complex. Upon Notch receptor activation and nuclear translocation of the Notch intracellular domain (NICD), MAML1 binds NICD and the transcription factor CSL (CBF1/RBPJ), forming a ternary complex that recruits additional coactivators to initiate target gene transcription.

MAML1 contains an N-terminal basic domain responsible for binding NICD and CSL, a central acidic transcriptional activation domain, and a C-terminal domain that mediates interactions with additional transcription factors and coactivators such as p300/CBP. Through these interactions, MAML1 promotes transcription of Notch target genes including HES1, HEY1, and MYC, which are involved in cell differentiation and proliferation. Because Notch signaling is crucial for tissue development and homeostasis, MAML1 serves as a master regulator of developmental pathways across multiple cell types, including neural, hematopoietic, and epithelial lineages.

The MAML1 antibody is widely used in molecular biology, developmental biology, and cancer research to assess Notch pathway activation and transcriptional regulation. Western blot analysis typically identifies a 115 kilodalton band corresponding to MAML1, while immunofluorescence reveals nuclear localization that overlaps with Notch pathway components. This antibody enables the study of transcriptional coactivation events and gene regulation in Notch-mediated signaling contexts.

Beyond Notch signaling, MAML1 also functions as a coactivator for other transcriptional regulators such as MEF2C, p53, and beta-catenin, broadening its impact on gene networks related to cell growth and apoptosis. Dysregulation of MAML1 has been linked to oncogenic transformation, particularly in leukemia, glioma, and breast cancer, where aberrant Notch signaling drives uncontrolled proliferation. The MAML1 antibody provides a reliable tool for investigating transcriptional complexes and signaling crosstalk mechanisms. NSJ Bioreagents offers this antibody validated for its applications, ensuring reproducibility and specificity for transcriptional and signaling studies.

UniProt: Q92585

Pathways: Notch Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development