TFDP3 Antikörper (AA 1-305)

Produktnummer ABIN7869171

Der Kaninchen Polyklonal anti-TFDP3 Antikörper wird verwendet zum Nachweis von TFDP3 in Proben von Human. Er wurde validiert für ELISA, WB, IHC, IF und ICC.

Kurzübersicht für TFDP3 Antikörper (AA 1-305) (ABIN7869171)

Target: TFDP3 (Transcription Factor Dp Family, Member 3 (TFDP3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TFDP3 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-TFDP3 Antikörper

Bindungsspezifität: AA 1-305

Verwendungszweck: TFDP3 Antibody / Transcription factor Dp family member 3

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TFDP3 recombinant protein (Position: M1-E305) was used as the immunogen for the TFDP3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TFDP3 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TFDP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TFDP3

Target: TFDP3 (Transcription Factor Dp Family, Member 3 (TFDP3))

Andere Bezeichnung: TFDP3

Hintergrund: TFDP3 antibody recognizes Transcription factor Dp family member 3, a unique atypical DP protein that modulates E2F-dependent transcription and cell cycle regulation. Unlike canonical DP proteins (TFDP1 and TFDP2), TFDP3 acts as a transcriptional repressor rather than an activator. It binds to E2F transcription factors but lacks the transactivation domain necessary for promoting gene expression, thereby acting as a competitive inhibitor. The TFDP3 antibody is an essential tool for studying cell proliferation, differentiation, apoptosis, and oncogenic transformation regulated through the E2F/DP pathway.

TFDP3 is encoded by the TFDP3 gene located on human chromosome Xq26.2. The protein consists of a conserved DNA-binding domain and a dimerization region that facilitates complex formation with E2F1. This heterodimerization blocks the transcriptional activation of E2F target genes involved in DNA synthesis and cell cycle progression. As a result, TFDP3 contributes to cell cycle arrest and resistance to apoptosis, especially under stress or DNA damage conditions.

The TFDP3 antibody is used to detect the 45-47 kDa nuclear protein in various cell lines and tissues. Immunofluorescence reveals nuclear localization consistent with its function as a transcriptional regulator. TFDP3 is predominantly expressed in testis, certain stem cells, and a subset of cancer tissues. Its overexpression has been reported in hepatocellular carcinoma, lung adenocarcinoma, and breast cancer, where it promotes survival by suppressing E2F1-induced apoptosis. Mechanistically, TFDP3 interferes with E2F1's ability to induce p53-independent cell death, thereby facilitating oncogenic proliferation.

Beyond cell cycle regulation, TFDP3 participates in differentiation processes and developmental gene expression control. It is also implicated in DNA repair regulation and stress-induced transcriptional reprogramming. Studies have shown that silencing TFDP3 enhances the cytotoxic effects of chemotherapeutic agents, making it a potential therapeutic target. NSJ Bioreagents offers a validated TFDP3 antibody optimized for western blot, immunohistochemistry, and chromatin immunoprecipitation, providing researchers with a robust reagent for dissecting E2F pathway regulation and its role in tumor biology.

UniProt: Q5H9I0

Pathways: Zellzyklus