NEK7 Antikörper (AA 1-302)

Produktnummer ABIN7869166

Der Kaninchen Polyklonal anti-NEK7 Antikörper (ABIN7869166) detektiert spezifisch NEK7 in WB, ELISA, IF, ICC, IP und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für NEK7 Antikörper (AA 1-302) (ABIN7869166)

Target: NEK7

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-NEK7 Antikörper

Bindungsspezifität: AA 1-302

Verwendungszweck: NEK7 Antibody / NIMA related kinase 7

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human NEK7 recombinant protein (Position: M1-S302) was used as the immunogen for the NEK7 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the NEK7 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the NEK7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu NEK7

Target: NEK7

Andere Bezeichnung: NEK7

Hintergrund: NEK7 antibody detects NIMA related kinase 7, encoded by the NEK7 gene. NIMA related kinase 7 is a serine/threonine protein kinase belonging to the NIMA related kinase family, which plays important roles in mitotic progression, spindle assembly, and cytokinesis. NEK7 antibody provides researchers with a specific reagent for studying cell cycle regulation, mitotic control, and disease processes associated with abnormal kinase activity.

NIMA related kinase 7 is expressed in proliferating tissues and localizes to the centrosome during mitosis. Research using NEK7 antibody has shown that it regulates microtubule organization, spindle formation, and chromosome segregation. By phosphorylating structural and regulatory proteins, NEK7 ensures orderly mitotic progression and completion of cytokinesis.

Studies with NEK7 antibody have also revealed a role in inflammasome activation. NEK7 interacts with NLRP3 and is essential for assembly of the NLRP3 inflammasome, which mediates caspase-1 activation and interleukin-1 beta processing. This discovery links NEK7 to innate immunity and inflammation, broadening its functional scope beyond mitosis.

Dysregulation of NEK7 has been implicated in cancer and inflammatory disease. Research using NEK7 antibody has shown that overexpression promotes proliferation and survival in certain cancers, while loss-of-function impairs mitotic fidelity and contributes to chromosomal instability. In immune contexts, defective NEK7 regulation disrupts inflammasome activity, contributing to autoinflammatory syndromes.

NEK7 antibody is commonly used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting quantifies kinase levels in proliferative tissues, immunohistochemistry highlights centrosomal and tumor expression, and immunofluorescence reveals dynamic localization during mitosis. These applications make NEK7 antibody indispensable for cell cycle and inflammation research.

By providing validated NEK7 antibody reagents, NSJ Bioreagents supports studies into mitosis, innate immunity, and cancer biology. Detection of NIMA related kinase 7 provides researchers with insight into how kinases regulate both cell division and immune signaling.

UniProt: Q8TDX7

Pathways: Inflammasome