TIGAR Antikörper (AA 1-270)

Produktnummer ABIN7869019

Der Kaninchen Polyklonal anti-TIGAR Antikörper (ABIN7869019) detektiert spezifisch TIGAR in WB, ELISA, IHC, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für TIGAR Antikörper (AA 1-270) (ABIN7869019)

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TIGAR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-TIGAR Antikörper

Bindungsspezifität: AA 1-270

Verwendungszweck: TIGAR Antibody / TP53-induced glycolysis and apoptosis regulator

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human TIGAR recombinant protein (Position: M1-R270) was used as the immunogen for the TIGAR antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TIGAR antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the TIGAR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu TIGAR

Target: TIGAR (TP53 induced glycolysis regulatory phosphatase (TIGAR))

Andere Bezeichnung: TIGAR

Hintergrund: TIGAR antibody detects TP53-induced glycolysis and apoptosis regulator, a key metabolic enzyme that modulates glycolysis, antioxidant defense, and cell survival. Encoded by the TIGAR gene on chromosome 12p13.3, this protein was identified as a transcriptional target of the tumor suppressor p53. TIGAR functions as a fructose-2,6-bisphosphatase, reducing levels of fructose-2,6-bisphosphate in the cytoplasm and thereby inhibiting glycolysis while promoting the pentose phosphate pathway. This metabolic shift enhances NADPH production, which supports antioxidant defense and protects cells against reactive oxygen species (ROS).

TIGAR contains a bisphosphatase domain homologous to that of the PFKFB family of enzymes but functions independently to regulate metabolic flux. By lowering intracellular fructose-2,6-bisphosphate, it decreases glycolytic activity and reduces lactate production while increasing glucose utilization for nucleotide synthesis and redox balance. This activity allows TIGAR to protect cells from oxidative stress and apoptosis under p53-mediated stress responses. It also contributes to autophagy regulation by balancing energy production and redox homeostasis.

The TIGAR antibody is used in metabolism, cancer, and redox biology research to investigate metabolic reprogramming and p53-dependent signaling. Western blot analysis typically identifies a 30 kilodalton band corresponding to TIGAR, while immunofluorescence shows cytoplasmic and perinuclear staining in metabolically active cells. Because TIGAR regulates the balance between glycolysis and antioxidant pathways, this antibody is a valuable tool for studying stress responses, metabolic adaptation, and tumor metabolism.

In cancer, TIGAR overexpression can support tumor cell survival by reducing oxidative stress, contributing to chemoresistance and metabolic flexibility. Conversely, TIGAR deficiency enhances ROS accumulation and apoptosis, sensitizing cells to stress. NSJ Bioreagents provides the TIGAR antibody validated for its applications, ensuring consistent detection in studies of metabolism, apoptosis, and cancer biology.

UniProt: Q9NQ88

Pathways: Warburg Effekt