PDX1 Antikörper (pan)

Produktnummer ABIN7868159

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PDX1 in WB, ELISA, IF, FACS und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PDX1 Antikörper (pan) (ABIN7868159)

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-PDX1 Antikörper

Bindungsspezifität: AA 1-145, pan

Verwendungszweck: PDX1 Antibody / Pancreatic and duodenal homeobox 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human PDX1 recombinant protein (Position: M1-E145) was used as the immunogen for the PDX1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the PDX1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the PDX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu PDX1

Target: PDX1 (Pancreatic and Duodenal Homeobox 1 (PDX1))

Andere Bezeichnung: PDX1

Hintergrund: PDX1 antibody detects Pancreatic and duodenal homeobox 1, a transcription factor that regulates pancreatic development, beta-cell differentiation, and insulin gene expression. The UniProt recommended name is Pancreatic and duodenal homeobox 1 (PDX1). This nuclear protein functions as a master regulator of pancreatic organogenesis and endocrine function, orchestrating the transcriptional networks that specify pancreatic progenitor cells and maintain mature beta-cell identity.

Functionally, PDX1 antibody identifies a 283-amino-acid homeodomain-containing protein that binds to promoter regions of multiple pancreatic genes, including insulin, somatostatin, glucokinase, and GLUT2. PDX1 interacts with transcriptional cofactors such as PBX1, NEUROD1, and MAFA to activate insulin transcription and coordinate glucose-stimulated insulin secretion. During embryogenesis, PDX1 marks multipotent progenitors in the foregut endoderm that give rise to all pancreatic cell lineages.

The PDX1 gene is located on chromosome 13q12.2 and is highly expressed in the pancreas, particularly in beta cells of the islets of Langerhans and in the duodenal epithelium. PDX1 expression is regulated by glucose, nutrient status, and signaling pathways including PI3K/AKT and MAPK. Its activity ensures proper glucose homeostasis and metabolic regulation.

Pathologically, mutations in PDX1 cause pancreatic agenesis, maturity-onset diabetes of the young type 4 (MODY4), and contribute to type 2 diabetes through impaired beta-cell function. Loss of PDX1 expression leads to beta-cell dedifferentiation, while overexpression can promote transdifferentiation of non-beta pancreatic cells into insulin-producing phenotypes. Research using PDX1 antibody supports studies in diabetes, pancreatic development, and regenerative medicine.

PDX1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect transcription factors involved in endocrine development. NSJ Bioreagents provides PDX1 antibody reagents optimized for studies in pancreatic biology, transcriptional regulation, and diabetes research.

Structurally, Pancreatic and duodenal homeobox 1 contains a highly conserved homeobox domain responsible for DNA binding and nuclear localization signals that target it to the nucleus. Post-translational modifications such as phosphorylation modulate PDX1 stability and transcriptional activity. This antibody enables investigation of PDX1's role in pancreatic morphogenesis, beta-cell identity maintenance, and metabolic gene regulation.

UniProt: P52945

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Hormone Transport, Carbohydrate Homeostasis, Chromatin Binding, Maintenance of Protein Location