IFT20 Antikörper (AA 1-132)

Produktnummer ABIN7868098

Der Kaninchen Polyklonal anti-IFT20 Antikörper (ABIN7868098) detektiert spezifisch IFT20 in WB, ELISA, IHC, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für IFT20 Antikörper (AA 1-132) (ABIN7868098)

Target: IFT20 (Intraflagellar Transport 20 Homolog (IFT20))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFT20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-IFT20 Antikörper

Bindungsspezifität: AA 1-132

Verwendungszweck: IFT20 Antibody / Intraflagellar transport protein 20 homolog

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human IFT20 recombinant protein (Position: M1-K132) was used as the immunogen for the IFT20 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the IFT20 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the IFT20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu IFT20

Target: IFT20 (Intraflagellar Transport 20 Homolog (IFT20))

Andere Bezeichnung: IFT20

Hintergrund: IFT20 antibody detects Intraflagellar transport protein 20 homolog, a component of the intraflagellar transport (IFT) complex required for cilium assembly, maintenance, and cargo trafficking. The UniProt recommended name is Intraflagellar transport protein 20 homolog (IFT20). This small protein serves as a crucial adaptor that links vesicular trafficking machinery to the IFT complex, ensuring proper delivery of membrane and signaling components to the cilium.

Functionally, IFT20 antibody identifies a 132-amino-acid protein localized to the Golgi apparatus and the base of cilia. IFT20 associates with the IFT-B complex and interacts with proteins such as IFT57, IFT88, and GMAP210 to coordinate transport of ciliary cargo from the Golgi to the basal body. It plays essential roles in ciliogenesis, ciliary membrane protein sorting, and Hedgehog signaling. IFT20 functions both in ciliated cells, such as renal epithelial cells and photoreceptors, and in non-ciliated cells, where it contributes to vesicle trafficking and immune synapse formation.

The IFT20 gene is located on chromosome 17q25.3 and is ubiquitously expressed in tissues with active ciliary assembly, including kidney, lung, retina, and brain. Expression is dynamically regulated during cell cycle progression and development, reflecting its importance in signaling and organelle biogenesis.

Pathologically, mutations or loss of IFT20 disrupt cilium formation and function, leading to a spectrum of ciliopathies such as polycystic kidney disease, retinal degeneration, and developmental disorders. Altered expression may also affect immune function, as IFT20 participates in T-cell receptor recycling. Research using IFT20 antibody supports studies in cilia biology, vesicular trafficking, and developmental signaling pathways.

IFT20 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary transport proteins. NSJ Bioreagents provides IFT20 antibody reagents optimized for studies in ciliogenesis, Golgi-cilium communication, and membrane trafficking.

Structurally, Intraflagellar transport protein 20 homolog is a small coiled-coil protein that integrates into the IFT-B complex. Its N-terminal region anchors it to the Golgi, while the C-terminal region mediates interactions with IFT components. This structural configuration enables IFT20 to couple vesicular transport and ciliary assembly. This antibody aids in investigating IFT20's role in cellular signaling, ciliogenesis, and trafficking fidelity.

UniProt: Q8IY31

Pathways: Hedgehog Signalweg