MYOD1 Antikörper (AA 1-120)

Produktnummer ABIN7868018

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch MYOD1 in WB und ELISA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für MYOD1 Antikörper (AA 1-120) (ABIN7868018)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-MYOD1 Antikörper

Bindungsspezifität: AA 1-120

Verwendungszweck: MYOD1 Antibody / Myoblast determination protein 1

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human MYOD1 recombinant protein (Position: M1-R120) was used as the immunogen for the MYOD1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MYOD1 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MYOD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MYOD1

Hintergrund: MYOD1 antibody detects Myoblast determination protein 1, a master transcription factor that controls skeletal muscle differentiation. The UniProt recommended name is Myoblast determination protein 1 (MYOD1). This basic helix-loop-helix (bHLH) transcription factor binds DNA at E-box motifs to activate muscle-specific gene expression, converting multipotent mesodermal cells into myoblasts.

Functionally, MYOD1 antibody identifies a 320-amino-acid nuclear protein that dimerizes with E proteins to regulate the transcription of myogenic regulatory genes such as MYOG and MYF5. MYOD1 acts as a pioneer factor, remodeling chromatin to establish muscle-specific transcriptional programs. It is indispensable for myogenic lineage commitment and muscle regeneration following injury.

The MYOD1 gene is located on chromosome 11p15.1 and is expressed in skeletal muscle progenitor cells and differentiating myoblasts. MYOD1 operates in coordination with MEF2 family transcription factors to control muscle fiber formation and repair. During development, its expression defines the onset of the myogenic program.

Pathologically, MYOD1 dysregulation is associated with muscle developmental disorders and certain sarcomas. Mutations in MYOD1 have been identified in rhabdomyosarcoma, where aberrant transcriptional activation promotes tumorigenesis. Research using MYOD1 antibody supports studies in muscle development, differentiation, and cancer biology.

MYOD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect myogenic transcription factors. NSJ Bioreagents provides MYOD1 antibody reagents optimized for skeletal muscle biology, stem cell differentiation, and gene regulation research.

Structurally, Myoblast determination protein 1 contains a bHLH domain that mediates DNA binding and dimerization. Its N-terminal activation domain recruits chromatin remodeling complexes to activate transcription. This antibody facilitates detailed examination of MYOD1's regulatory network in muscle cell fate determination.

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development