Feimin Antikörper

Produktnummer ABIN7866182

Der Maus Monoklonal anti-Feimin Antikörper wird verwendet zum Nachweis von Feimin in Proben von Maus und Human. Er wurde validiert für ELISA, IP, WB und SDS.

Kurzübersicht für Feimin Antikörper (ABIN7866182)

Target: Feimin

Reaktivität: Maus, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB), SDS-PAGE (SDS)

Produktdetails

Verwendungszweck: Anti-Feimin (MOUSE) Monoclonal Antibody

Kreuzreaktivität (Details): A BLAST analysis of the sequence of the immunogen shows 100% identity with mouse, 97% bovine, and 94% human. This product reacts with mouse, and human Feimin isoform 1 and 2. Reactivity against homologues from other sources is not known.

Aufreinigung: Anti-Feimin Monoclonal Antibody is Protein A purified

Sterilität: Sterile filtered

Immunogen: This monoclonal antibody was prepared in mice after repeated immunizations with recombinant mouse Feimin protein.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Anti-Feimin (MOUSE) Antibody has been tested by ELISA, Western Blot, and Immunoprecipitation. The antibody detects both human and mouse feimin protein from overexpressing cell lysates (using HEK293T). A band approximately 17kDa for isoform 2 and 20kDa fo

Kommentare:

ELISA Dilution: 1:265,000-1:409,000
Western Blot Dilution: 41:5000br />Immunoprecipitation Dilution: 40 ng/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.16 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for

Haltbarkeit: 12 months

Antigendetails

Target: Feimin

Hintergrund: Background: The Feimin protein, also known as UPF0461 protein or C5orf24, involves regulatory roles within the cell and its exact role is under investigation. Feimin is a newly discovered myokine that complements insulin by fine-tuning glucose levels and boosting exercise capacity, especially under conditions where insulin is less effective. Highly conserved Feimin functions in both glucose homeostasis and has a direct role in muscle thermogenesis. Feimin can be secreted by muscle and act as a myokine where it can affect tissues by engaging MERTK receptor thereby activating AKT. Cellular Feimin (cFeimin) functions as a transcription regulator by interacting with FOXC2 protein, where it helps to enhance exercise performance by reducing thermogenesis.
Synonyms: Chromosome 5 Open Reading Frame 24, UPF0461 Protein C5orf24, FLJ37562, Feimin

Gen-ID: 134553

NCBI Accession: NP_001129058.1

UniProt: Q80X32