CD2 Antikörper

Produktnummer ABIN7866122

Der Maus Monoklonal anti-CD2 Antikörper wird verwendet zum Nachweis von CD2 in Proben von Human. Er wurde validiert für FACS.

Kurzübersicht für CD2 Antikörper (ABIN7866122)

Target: CD2

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: T6-3

Produktdetails anti-CD2 Antikörper

Verwendungszweck: Mouse anti Human CD2

Produktmerkmale: Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.

Aufreinigung: Protein A/G Chromatography

Immunogen: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: PBMC: Add 10 μL of antibody to 1 million PBMCs in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 μL of antibody to 100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for immunofluorescence analysis with a flow cytometer or microscope.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu CD2

Target: CD2

Andere Bezeichnung: CD2

Hintergrund: T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein

UniProt: P06729