UACA Antikörper

Produktnummer ABIN7865752

Der Maus Monoklonal anti-UACA Antikörper (Klon AE-5) (ABIN7865752) detektiert spezifisch UACA in WB, IF, FACS, IHC und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für UACA Antikörper (ABIN7865752)

Target: UACA (Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UACA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: AE-5

Produktdetails anti-UACA Antikörper

Verwendungszweck: anti-UACA mouse monoclonal, AE-5, purified

Aufreinigung: affinity chromatography

Immunogen: nuclei of myeloid leukemia biopsy cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu UACA

Target: UACA (Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA))

Andere Bezeichnung: UACA

Hintergrund: UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, a mRNA differentially expressed in F9 embryonal carcinoma cells, and that is up-regulated during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway. Positive control: HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.

Pathways: Positive Regulation of Endopeptidase Activity