S100A9 Antikörper

Produktnummer ABIN7865729

Dieser Maus Monoklonal Antikörper detektiert spezifisch S100A9 in WB, IHC, IF, ICC und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Hund, Schwein, Meerschweinchen, Pferd, Kaninchen, Cat, Ziege und Affe.

Kurzübersicht für S100A9 Antikörper (ABIN7865729)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reaktivität: Human, Ratte, Hund, Schwein, Meerschweinchen, Pferd, Kaninchen, Cat, Ziege, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 47-8D3

Produktdetails anti-S100A9 Antikörper

Verwendungszweck: anti-MRP14/S100A9/calgranulin B mouse monoclonal, 47-8D3, purified

Aufreinigung: affinity chromatography

Immunogen: human peripheral blood monocyte components derived by affinity chromatography with Sepharose 4B coupled to rabbit monocyte antibodies

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/mL PBS recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Andere Bezeichnung: MRP14/S100A9/calgranulin B

Hintergrund: 47-8D3 reacts with macrophages and detects the well-known leukocyte L1, cystic fibrosis antigen. Detecting a single protein band of 14 kDa in Western blots of lysates of human monocytes and granulocytes, the antigen was identified as the calcium-binding protein MRP14, which is a member of the S100 family involved a.o. in regulating the cell cycle. MRP14 is also implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. It is further found on squamous mucosal epithelia. When associated with MRP8 it forms the heterodimer calprotectin. Positive control: Monocytes, macrophages, granulocytes.

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine