Retinol-Binding Protein Antikörper

Produktnummer ABIN7865727

Der Maus Monoklonal anti-Retinol-Binding Protein Antikörper wird verwendet zum Nachweis von Retinol-Binding Protein in Proben von Maus, Ziege, Affe, Ratte und Kaninchen. Er wurde validiert für IHC, WB und ELISA.

Kurzübersicht für Retinol-Binding Protein Antikörper (ABIN7865727)

Target: Retinol-Binding Protein (RBP)

Reaktivität: Maus, Ziege, Affe, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Retinol-Binding Protein Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Klon: G4E4

Produktdetails anti-Retinol-Binding Protein Antikörper

Verwendungszweck: anti-Retinol Binding Protein mouse monoclonal, G4E4, purified

Aufreinigung: affinity chromatography

Immunogen: with human retinol binding protein purified from plasma

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:100-1:200 (0.5-1 μg/mL)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

elisa_dilution: assay dependent

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu Retinol-Binding Protein

Target: Retinol-Binding Protein (RBP)

Andere Bezeichnung: Retinol Binding Protein

Hintergrund: G4E4 recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver. Positive control: Liver.