NUMA1 Antikörper

Produktnummer ABIN7865708

Dieser Maus Monoklonal Antikörper detektiert spezifisch NUMA1 in WB, IHC und ELISA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für NUMA1 Antikörper (ABIN7865708)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NUMA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: EBS-C-002

Produktdetails anti-NUMA1 Antikörper

Verwendungszweck: anti-NuMA mouse monoclonal, EBS-C-002, purified

Aufreinigung: affinity chromatography

Immunogen: live Ls 174T cells (colon carcinoma)

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: store at 2 - 8 °C

Details zu NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Andere Bezeichnung: NuMA

Hintergrund: EBS-C-002 reacts with NuMA or Nuclear Mitotic Apparatus protein, which at the onset of mitosis redistributes from the nucleus to two centrosomal structures at the poles of the mitotic spindle, where it plays a vital role in establishing and maintaining its bipolar structure. After anaphase the protein redistributes from the spindle polar region into the reforming nucleus and concentrates initially at the site where nuclear lamins and perichomatin have been reported to assemble. In contrast to mitotic cells, post-mitotic neurons display NuMA both in the nucleus and in the cytoplasm. Due to release from dead cells, NuMA is also used as oncological marker in serum and urine. In addition, chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia. Positive control: Human toncil.

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, M Phase