CD45RA Antikörper
Kurzübersicht für CD45RA Antikörper (ABIN7865593)
Target
Alle CD45RA Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- anti-CD45RA mouse monoclonal, 158-4D3, purified
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Aufreinigung
- affinity chromatography
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Immunogen
- stimulated human leucocytes
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Isotyp
- IgG2a kappa
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Applikationshinweise
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- store at 2 - 8 °C
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- CD45RA
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Andere Bezeichnung
- CD45RA
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Hintergrund
- CD45 glycoprotein have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cell multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45 RB (exon 5, CD45RC (exon 6) and CD45R or CD45RO (exons 4-6 spliced out). 158-4D3 was clustered in Boston at the Vth International CD workshop as anti CD45RA, useful to differentiate "naive" T-cells from activated (CD45R0+ve) T-cells, or to differentiate T-cell lymphomas (CD45R0+ve) from B-cell lymphomas (CD45RA+ve). Positive control: Daudi, Raji, IM9 and human lymphocytes. Human lymph node and tonsil.
Target
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