Rekombinanter Vimentin Antikörper

Produktnummer ABIN7863500

Der Kaninchen Monoklonal anti-Vimentin Antikörper wird verwendet zum Nachweis von Vimentin in Proben von Human, Schwein, Rind (Kuh), Huhn, Hund, Cat und Pferd. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter Vimentin Antikörper (ABIN7863500)

Target: Vimentin (VIM)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Pferd

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: VIM-6576R

Produktdetails anti-Vimentin Antikörper

Verwendungszweck: Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

Spezifität: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Vimentin protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund:

VIM

A549, Raji, U87, Jurkat or HeLa cells. Human lymph node or tonsil.

Molekulargewicht: 57-60kDa

Gen-ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose