Rekombinanter SPARC Antikörper (C-Term)

Produktnummer ABIN7865427

Der Maus Monoklonal anti-SPARC Antikörper wird verwendet zum Nachweis von SPARC in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter SPARC Antikörper (C-Term) (ABIN7865427)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: ROSTN-9835

Produktdetails anti-SPARC Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Recombinant SPARC / Osteonectin (O-Nectin) Antibody

Spezifität: SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment corresponding to the C-terminus of human SPARC protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Andere Bezeichnung: SPARC

Hintergrund:

Basement membrane protein 40, BM40, Cysteine rich protein, OI17, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and cysteine rich, Secreted protein acidic and rich in cysteine (SPARC)

Human placenta, lymph node or spleen. JEG3, A-375 or A549 cell lysates.

Molekulargewicht: 38kDa

Gen-ID: 6678, 111779, 708558

UniProt: P09486

Pathways: Autophagie