MCM4 Antikörper (AA 600-800)

Produktnummer ABIN7865407

Dieser Maus Monoklonal Antikörper detektiert spezifisch MCM4 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für MCM4 Antikörper (AA 600-800) (ABIN7865407)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: MCM4-3041

Produktdetails anti-MCM4 Antikörper

Bindungsspezifität: AA 600-800

Verwendungszweck: MCM4 (Pan Cancer Marker) Antibody

Spezifität: The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double- stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-≥-induced Stat1± transcription activation.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa600-800) of human MCM4 protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4

Hintergrund:

NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21

A-431 cells. Human colon.

Molekulargewicht: 100kDa

Gen-ID: 4173, 460184

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA