LAG3 Antikörper (AA 300-500)

Produktnummer ABIN7863779

Der Maus Monoklonal anti-LAG3 Antikörper wird verwendet zum Nachweis von LAG3 in Proben von Human. Er wurde validiert für IHC.

Kurzübersicht für LAG3 Antikörper (AA 300-500) (ABIN7863779)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LAG3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: LAG3-7380

Produktdetails anti-LAG3 Antikörper

Bindungsspezifität: AA 300-500

Verwendungszweck: Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator) Antibody

Spezifität: LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 Molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved KIEELE motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa300-500) of human LAG3 protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Andere Bezeichnung: Lymphocyte Activation Gene 3

Hintergrund:

CD223, FDC protein, LAG-3

Human tonsil or Hodgkin s lymphoma (IHC-P).

Molekulargewicht: 70kDa

Gen-ID: 3902, 409523

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints