Rekombinanter Vimentin Antikörper (AA 2-466)
Kurzübersicht für Rekombinanter Vimentin Antikörper (AA 2-466) (ABIN7863499)
Target
Alle Vimentin (VIM) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 2-466
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Verwendungszweck
- Recombinant Vimentin (Mesenchymal Cell Marker) Antibody
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Spezifität
- This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
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Aufreinigung
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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Immunogen
- Recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)
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Isotyp
- IgG, kappa
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- Vimentin (VIM)
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Andere Bezeichnung
- Vimentin
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Hintergrund
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VIM
Raji, U-87, Jurkat or HeLa cells. Human lymph node or tonsil. A549, U-87, Human Skeletal muscle. Lung, Skin.
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Molekulargewicht
- 57-60kDa
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Gen-ID
- 7431, 455493
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UniProt
- P08670
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Pathways
- Caspase Kaskade in der Apoptose
Target
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