IRS1 Antikörper (AA 1-200)

Produktnummer ABIN7858544

Dieser Maus Monoklonal Antikörper detektiert spezifisch IRS1 in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für IRS1 Antikörper (AA 1-200) (ABIN7858544)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IRS1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: IRS1-7571

Produktdetails anti-IRS1 Antikörper

Bindungsspezifität: AA 1-200

Verwendungszweck: IRS1 (Insulin receptor substrate 1) Antibody

Spezifität: The Insulin receptor substrate-1 (IRS-1), a protein major substrate of the Insulin receptor, is phosphorylated in response to stimulation of cells by Insulin, Insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An Insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the Insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase IIcatalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase IIcatalyzed phosphorylation of IRS-1 may be a component of the intracellular Insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the Insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of Insulin sensitivity by interrupting the association between the Insulin receptor and IRS-1.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human IRS1 protein (exact sequence is proprietary)

Isotyp: IgG2, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Andere Bezeichnung: IRS1

Hintergrund:

HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1, IRS1_HUMAN, OTTHUMP00000164234

adrenal gland or lung.|Human breast carcinoma

Molekulargewicht: 170-185kDa

Gen-ID: 3667, 445868

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process