Caspase 3 Antikörper (AA 1-200)

Produktnummer ABIN7858374

Der Maus Monoklonal anti-Caspase 3 Antikörper wird verwendet zum Nachweis von Caspase 3 in Proben von Human. Er wurde validiert für IHC.

Kurzübersicht für Caspase 3 Antikörper (AA 1-200) (ABIN7858374)

Target: Caspase 3 (CASP3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: CASP3-8446

Produktdetails anti-Caspase 3 Antikörper

Bindungsspezifität: AA 1-200

Verwendungszweck: Caspase 3 (Pro & Active Form) Antibody

Spezifität: This MAb recognizes a protein of 32 kDa, identified as Caspase 3. It is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp 175-Ser 176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp 28-Ser 29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 sub- units. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at a Asp 216-Gly 217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human CASP3 protein (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Details zu Caspase 3

Target: Caspase 3 (CASP3)

Andere Bezeichnung: Caspase 3

Hintergrund:

APOPAIN, Apoptosis related cysteine peptidase, CASP3, Caspase3, CPP32, CPP32B, cysteine protease CPP32, PARP cleavage protease, Procaspase3, SREBP cleavage activity 1 (SCA1), Yama

Heart|HL60 or Jurkat cells. Human liver|liver or kidney.|spleen

Molekulargewicht: 32kDa

Gen-ID: 836, 141125

UniProt: P42574

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation