H2AFX Antikörper (pSer140) (Cy7)

Produktnummer ABIN742544

Der Kaninchen Polyklonal Anti-H2AFX-Antikörper wurde für WB, FACS, IF (p) und IF (cc) validiert. Er ist geeignet, H2AFX in Proben von Human zu detektieren.

Kurzübersicht für H2AFX Antikörper (pSer140) (Cy7) (ABIN742544)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser H2AFX Antikörper ist konjugiert mit Cy7

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Produktdetails anti-H2AFX Antikörper (Cy7)

Bindungsspezifität: pSer140

Spezifität: This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Andere Bezeichnung: Histone H2a.X

Hintergrund:

Synonyms: H2AX, H2A.X, H2A/X, Histone H2AX, Histone H2A.X, H2AFX

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Gen-ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus