H2AFX Antikörper (pSer140)

Produktnummer ABIN742538

Der Kaninchen Polyklonal Anti-H2AFX-Antikörper wurde für WB, ELISA, IHC (p), ICC, FACS, IF (p), IF (cc) und IHC (fro) validiert. Er ist geeignet, H2AFX in Proben von Human zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für H2AFX Antikörper (pSer140) (ABIN742538)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser H2AFX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-H2AFX Antikörper

Bindungsspezifität: pSer140

Spezifität: This phosphorylation site is homologous to that of Ser140 in Mouse and Rat.

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-H2AFX Antikörper (ABIN742538)

Chen, Song, Hou, Li: "Reactive oxygen species induced by icaritin promote DNA strand breaks and apoptosis in human cervical cancer cells." in: Oncology reports, Vol. 41, Issue 2, pp. 765-778, (2019) (PubMed).

Shi, Wang, Li, Bai, Li, Li, Wang, Zhou: "Dihydroartemisinin induces autophagy-dependent death in human tongue squamous cell carcinoma cells through DNA double-strand break-mediated oxidative stress." in: Oncotarget, Vol. 8, Issue 28, pp. 45981-45993, (2018) (PubMed).

Song, Shi, Liu, Wang, Wang, Song, Song: "Unpredicted Downregulation of RAD51 Suggests Genome Instability Induced by Tetrachlorobenzoquinone." in: Chemical research in toxicology, Vol. 29, Issue 12, pp. 2184-2193, (2016) (PubMed).

Shi, Yang, Mao, Wu, Ren, Yang, Yin, Wei, Li, Wang: "Nutlin-3-induced redistribution of chromatin-bound IFI16 in human hepatocellular carcinoma cells in vitro is associated with p53 activation." in: Acta pharmacologica Sinica, Vol. 36, Issue 2, pp. 252-8, (2015) (PubMed).

Details zu H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Andere Bezeichnung: Histone H2a.X

Hintergrund:

Synonyms: H2AX, H2A.X, H2A/X, Histone H2AX, Histone H2A.X, H2AFX

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Gen-ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus