TCR beta Antikörper (PE-Cy5)

Produktnummer ABIN7845269

Der PE-Cy5-konjugierte Armenischer Hamster Monoklonal anti-TCR beta Antikörper (Klon H57-597) (ABIN7845269) detektiert spezifisch TCR beta in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für TCR beta Antikörper (PE-Cy5) (ABIN7845269)

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser TCR beta Antikörper ist konjugiert mit PE-Cy5

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: H57-597

Produktdetails anti-TCR beta Antikörper (PE-Cy5)

Verwendungszweck: Armenian hamster monoclonal antibody PE/Cy5 labled to TCR beta

Spezifität: Recognizes mouse TCR beta

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Affinity purified TCR from mouse DO-11.10 cells

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu TCR beta

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Andere Bezeichnung: TCRB

Hintergrund: Tib, TCRbeta

Gen-ID: 21577