TCRb Antikörper

Produktnummer ABIN7845264

Dieser Armenischer Hamster Monoklonal Antikörper detektiert spezifisch TCRb in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für TCRb Antikörper (ABIN7845264)

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser TCRb Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: H57-597

Produktdetails anti-TCRb Antikörper

Verwendungszweck: Armenian Hamster monoclonal antibody to TCR beta

Spezifität: Recognizes mouse TCR beta

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Affinity purified TCR from mouse DO-11.10 cells

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu TCRb

Target: TCRb (T Cell Receptor beta Chain (TCRb))

Andere Bezeichnung: TCRB

Hintergrund: Tib, TCRbeta

Gen-ID: 21577