IL12A Antikörper

Produktnummer ABIN7840011

Dieser Maus Monoklonal Antikörper detektiert spezifisch IL12A in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für IL12A Antikörper (ABIN7840011)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL12A Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 20-c2

Produktdetails anti-IL12A Antikörper

Verwendungszweck: Mouse monoclonal antibody to IL-12 p70

Spezifität: Recognizes human IL-12 p70

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Recombinant human IL-12 (p70)

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Andere Bezeichnung: IL12A

Hintergrund: NKSF1, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, NK cell stimulatory factor chain 1, NKSF1, NKSF2, Interleukin-12 subunit beta, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, NK cell stimulatory factor chain 2, NKSF2

Gen-ID: 3592

UniProt: P29459

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation