IFNAR1 Antikörper

Produktnummer ABIN7839936

Dieser Maus Monoklonal Antikörper detektiert spezifisch IFNAR1 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für IFNAR1 Antikörper (ABIN7839936)

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IFNAR1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: MAR1-5A3

Produktdetails anti-IFNAR1 Antikörper

Verwendungszweck: Mouse monoclonal antibody to IFNAR1

Spezifität: Recognizes mouse IFNAR1

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Exogenously expressed murine IFNAR1 extracellular domain

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu IFNAR1

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Andere Bezeichnung: IFNAR1

Hintergrund: IFNAR, Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1, CRF2-1, Type I interferon receptor 1

Gen-ID: 15975

Pathways: JAK-STAT Signalweg, Hepatitis C