HLA-DRA Antikörper (FITC)

Produktnummer ABIN7839676

Der Maus Monoklonal anti-HLA-DRA Antikörper wird verwendet zum Nachweis von HLA-DRA in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für HLA-DRA Antikörper (FITC) (ABIN7839676)

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-DRA Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: LN3

Produktdetails anti-HLA-DRA Antikörper (FITC)

Verwendungszweck: Mouse monoclonal antibody FITC labled to HLA-DR

Spezifität: Recognizes human HLA-DR

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human HLA-DR.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu HLA-DRA

Target: HLA-DRA (HLA Class II DR alpha (HLA-DRA))

Andere Bezeichnung: HLA-DRA

Hintergrund: HLA-DRA1, HLA class II histocompatibility antigen. DR alpha chain, MHC class II antigen DRA

Gen-ID: 3122

UniProt: P01903

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Human Leukocyte Antigen (HLA) in Adaptive Immune Response