CD8 alpha Antikörper (PerCP-Cy5.5)

Produktnummer ABIN7836612

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD8 alpha in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD8 alpha Antikörper (PerCP-Cy5.5) (ABIN7836612)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD8 alpha Antikörper ist konjugiert mit PerCP-Cy5.5

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: SK1

Produktdetails anti-CD8 alpha Antikörper (PerCP-Cy5.5)

Verwendungszweck: Mouse monoclonal antibody PerCP/Cy5.5 labled to CD8

Spezifität: Recognizes human CD8

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD8.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung: CD8A

Hintergrund: MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD antigen CD8a, CD8B1, T-cell surface glycoprotein CD8 beta chain, CD antigen CD8b

Gen-ID: 925

UniProt: P01732

Pathways: T-Zell Rezeptor Signalweg