CD73 Antikörper (APC)
Kurzübersicht für CD73 Antikörper (APC) (ABIN7836580)
Target
Alle CD73 (NT5E) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Mouse monoclonal antibody APC labled to CD73
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Spezifität
- Recognizes human CD73
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Aufreinigung
- The antibody was purified by affinity chromatography.
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Immunogen
- Native purified human CD73.
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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E Reaktivität: Human WB, IHC, ELISA Wirt: Maus Monoclonal 4G6B10 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibodyNT5E Reaktivität: Human, Maus WB, IHC (p) Wirt: Kaninchen Polyclonal RB02846 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibodyNT5E Reaktivität: Human WB, IHC, ICC, IP Wirt: Kaninchen Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E Reaktivität: Human WB, IHC, FACS, IF, ELISA Wirt: Maus Monoclonal NT5E-2545 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E Reaktivität: Human WB, IHC, FACS, IF Wirt: Maus Monoclonal NT5E-2503 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibodyNT5E Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E Reaktivität: Human WB, IHC, FACS, ELISA Wirt: Maus Monoclonal 4G6E3 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibodyNT5E Reaktivität: Rind (Kuh) WB, IHC, ICC, IP Wirt: Kaninchen Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)NT5E Reaktivität: Human, Non-Human Primate FACS Wirt: Maus Monoclonal AD2 PE
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibodyNT5E Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schaf WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
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Applikationshinweise
- Flow: 10 μL / assay
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Protokoll
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Shipped and store at 4°C for one year. Do not freeze.
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Andere Bezeichnung
- NT5E
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Hintergrund
- NT5, NTE, 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD antigen CD73
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Gen-ID
- 4907
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UniProt
- P21589
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Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
Target
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