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CD73 Antikörper

NT5E Reaktivität: Human WB, FACS, IHC, ELISA, IF, StM, Coat Wirt: Maus Monoclonal NT5E-2545 unconjugated
Produktnummer ABIN6940229
  • Target Alle CD73 (NT5E) Antikörper anzeigen
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Reaktivität
    • 119
    • 59
    • 27
    • 18
    • 18
    • 16
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 92
    • 39
    • 17
    • 2
    Maus
    Klonalität
    • 83
    • 67
    Monoklonal
    Konjugat
    • 62
    • 18
    • 14
    • 11
    • 10
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD73 Antikörper ist unkonjugiert
    Applikation
    • 95
    • 64
    • 59
    • 50
    • 19
    • 19
    • 14
    • 14
    • 14
    • 8
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    Recombinant full length human NT5E protein
    Klon
    NT5E-2545
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product NT5E Primärantikörper
  • Applikationshinweise

    Positive Control: Tonsil, Prostate or Lung Carcinoma.

    Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Haltbarkeit
    24 months
  • Target
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Andere Bezeichnung
    NT5E (NT5E Produkte)
    Synonyme
    CALJA antikoerper, CD73 antikoerper, E5NT antikoerper, NT antikoerper, NT5 antikoerper, NTE antikoerper, eN antikoerper, eNT antikoerper, zgc:63784 antikoerper, NT5E antikoerper, MGC114869 antikoerper, PSPTO2277 antikoerper, 2210401F01Rik antikoerper, AI447961 antikoerper, Nt5 antikoerper, 5'-nucleotidase ecto antikoerper, 5'-nucleotidase, ecto (CD73) antikoerper, 5'-nucleotidase antikoerper, 5' nucleotidase, ecto antikoerper, NT5E antikoerper, nt5e antikoerper, nt5e.S antikoerper, PSPTO_2277 antikoerper, CBU_1233 antikoerper, MCA1322 antikoerper, Nt5e antikoerper
    Hintergrund
    CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
    Molekulargewicht
    71kDa
    Gen-ID
    4907
    UniProt
    P21589
    Pathways
    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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