CD53 Antikörper (FITC)

Produktnummer ABIN7836450

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD53 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD53 Antikörper (FITC) (ABIN7836450)

Target: CD53

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD53 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: C53-2

Produktdetails anti-CD53 Antikörper (FITC)

Verwendungszweck: Mouse monoclonal antibody FITC labled to CD53

Spezifität: Recognizes human CD53

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD53.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53

Hintergrund: MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25, CD antigen CD53

Gen-ID: 963

UniProt: P19397