CD51 Antikörper (FITC)

Produktnummer ABIN7836446

Der FITC-konjugierte Maus Monoklonal anti-CD51 Antikörper (Klon 13C2) (ABIN7836446) detektiert spezifisch CD51 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD51 Antikörper (FITC) (ABIN7836446)

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD51 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 13C2

Produktdetails anti-CD51 Antikörper (FITC)

Verwendungszweck: Mouse monoclonal antibody FITC labled to CD51

Spezifität: Recognizes human CD51

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Cell suspension containing osteoclasts from osteoclastomas

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Andere Bezeichnung: ITGAV

Hintergrund: MSK8, VNRA, Integrin alpha-V, Vitronectin receptor subunit alpha, CD51

Gen-ID: 3685

UniProt: P06756

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex