ITGA2 Antikörper (PE)

Produktnummer ABIN7836409

Der Ratte Monoklonal anti-ITGA2 Antikörper wird verwendet zum Nachweis von ITGA2 in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für ITGA2 Antikörper (PE) (ABIN7836409)

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGA2 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: DX5

Produktdetails anti-ITGA2 Antikörper (PE)

Verwendungszweck: Rat monoclonal antibody APC labled to CD49b

Spezifität: Recognizes mouse CD49b

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Mouse colon carcinoma cell line Colon26

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Andere Bezeichnung: ITGA2

Hintergrund: CD49B, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, GPIa, VLA-2 subunit alpha, CD49b

Gen-ID: 16398

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex