CD47 Antikörper (PE)

Produktnummer ABIN7836401

Der PE-konjugierte Maus Monoklonal anti-CD47 Antikörper (Klon CC2C6D4) (ABIN7836401) detektiert spezifisch CD47 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CD47 Antikörper (PE) (ABIN7836401)

Target: CD47

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD47 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: CC2C6D4

Produktdetails anti-CD47 Antikörper (PE)

Verwendungszweck: Mouse monoclonal antibody PE labled to CD47

Spezifität: Recognizes human CD47

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD47.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD47

Target: CD47

Andere Bezeichnung: CD47

Hintergrund: MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD antigen CD47

Gen-ID: 961

UniProt: Q08722