CD47 Antikörper (APC)
Kurzübersicht für CD47 Antikörper (APC) (ABIN7836399)
Target
Alle CD47 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Rat monoclonal antibody APC labled to CD47
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Spezifität
- Recognizes mouse CD47
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Aufreinigung
- The antibody was purified by affinity chromatography.
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anti-CD47 (CD47) antibody
CD47 Reaktivität: Human FACS, WB, IF, Func Wirt: Maus Monoclonal B6H12-2 unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 Reaktivität: Human FACS, IHC, IF, ELISA Wirt: Maus Monoclonal CD47-3019 unconjugated
anti-CD47 (CD47) (AA 20-140) antibodyCD47 Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-219) antibodyCD47 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 Reaktivität: Human WB, IHC, ELISA Wirt: Maus Monoclonal CD47-2937 unconjugated
anti-CD47 (CD47) (AA 241-323) antibodyCD47 Reaktivität: Maus, Ratte FACS, WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-CD47 (CD47) antibody (FITC)CD47 Reaktivität: Human, Schwein, Non-Human Primate FACS Wirt: Maus Monoclonal MEM-122 FITC
anti-CD47 (CD47) antibodyCD47 Reaktivität: Human, Schwein, Non-Human Primate FACS, WB, IHC (fro) Wirt: Maus Monoclonal MEM-122 unconjugated
anti-CD47 (CD47) (AA 19-142) antibodyCD47 Reaktivität: Human WB, IHC, ICC Wirt: Kaninchen Monoclonal BLR131H unconjugated Recombinant Antibody
anti-CD47 (CD47) antibodyCD47 Reaktivität: Human WB, IHC, IF, ELISA Wirt: Kaninchen Monoclonal 4G11 unconjugated Recombinant Antibody
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Applikationshinweise
- Flow: 10 μL / assay
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Protokoll
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Buffer
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Shipped and store at 4°C for one year. Do not freeze.
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- CD47
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Andere Bezeichnung
- CD47
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Hintergrund
- MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47
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Gen-ID
- 16423
Target
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