ITGB1 Antikörper (PE)

Produktnummer ABIN7836089

Der PE-konjugierte Maus Monoklonal anti-ITGB1 Antikörper (Klon TS2-16-2-1) (ABIN7836089) detektiert spezifisch ITGB1 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für ITGB1 Antikörper (PE) (ABIN7836089)

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGB1 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: TS2-16-2-1

Produktdetails anti-ITGB1 Antikörper (PE)

Verwendungszweck: Mouse monoclonal antibody PE labled to CD29

Spezifität: Recognizes human CD29

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD29.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Andere Bezeichnung: ITGB1

Hintergrund: FNRB, MDF2, MSK12, Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD antigen CD29

Gen-ID: 3688

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interaktom