ICOS Antikörper (APC)

Produktnummer ABIN7836055

Der Armenischer Hamster Monoklonal anti-ICOS Antikörper wird verwendet zum Nachweis von ICOS in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für ICOS Antikörper (APC) (ABIN7836055)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reaktivität: Human

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser ICOS Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: C398-4A

Produktdetails anti-ICOS Antikörper (APC)

Verwendungszweck: Armenian Hamster monoclonal antibody APC labled to CD278

Spezifität: Recognizes human CD278

Aufreinigung: The antibody was purified by affinity chromatography.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Andere Bezeichnung: ICOS

Hintergrund: AILIM, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278

Gen-ID: 29851

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints