ICOS Antikörper

Produktnummer ABIN7836054

Dieser Armenischer Hamster Monoklonal Antikörper detektiert spezifisch ICOS in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für ICOS Antikörper (ABIN7836054)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reaktivität: Human, Maus, Ratte

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser ICOS Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: C398-4A

Produktdetails anti-ICOS Antikörper

Verwendungszweck: Armenian Hamster monoclonal antibody to CD278

Spezifität: Recognizes human/mouse/rat CD278

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Mouse T cell clone D10.G4.1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Andere Bezeichnung: ICOS

Hintergrund: AILIM, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278

Gen-ID: 29851, 54167, 64545

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints