CD24 Antikörper (PerCP-Cy5.5)

Produktnummer ABIN7835997

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD24 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD24 Antikörper (PerCP-Cy5.5) (ABIN7835997)

Target: CD24 (CD24 Molecule (CD24))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD24 Antikörper ist konjugiert mit PerCP-Cy5.5

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: SN3

Produktdetails anti-CD24 Antikörper (PerCP-Cy5.5)

Verwendungszweck: Mouse monoclonal antibody PerCP/Cy5.5 labled to CD24

Spezifität: Recognizes human CD24

Aufreinigung: The antibody was purified by affinity chromatography.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD24

Target: CD24 (CD24 Molecule (CD24))

Andere Bezeichnung: CD24

Hintergrund: CD24A, Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen, CD24

Gen-ID: 100133941

UniProt: P25063

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation