CD24 Antikörper (PE-Cy5)

Produktnummer ABIN7835996

Der PE-Cy5-konjugierte Ratte Monoklonal anti-CD24 Antikörper (Klon 91) (ABIN7835996) detektiert spezifisch CD24 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für CD24 Antikörper (PE-Cy5) (ABIN7835996)

Target: CD24 (CD24 Molecule (CD24))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD24 Antikörper ist konjugiert mit PE-Cy5

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 91

Produktdetails anti-CD24 Antikörper (PE-Cy5)

Verwendungszweck: Rat monoclonal antibody PE/Cy5 labled to CD24

Spezifität: Recognizes mouse CD24

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD24

Target: CD24 (CD24 Molecule (CD24))

Andere Bezeichnung: CD24

Hintergrund: CD24A, Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen, CD24

Gen-ID: 12484

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation