CD235a/GYPA Antikörper (PE)

Produktnummer ABIN7835986

Der Maus Monoklonal anti-CD235a/GYPA Antikörper wird verwendet zum Nachweis von CD235a/GYPA in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für CD235a/GYPA Antikörper (PE) (ABIN7835986)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD235a/GYPA Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: JC159

Produktdetails anti-CD235a/GYPA Antikörper (PE)

Verwendungszweck: Mouse monoclonal antibody PE labled to CD235a

Spezifität: Recognizes human CD235a

Aufreinigung: The antibody was purified by affinity chromatography.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Andere Bezeichnung: GYPA

Hintergrund: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD235a

Gen-ID: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location