CXCR3 Antikörper (N-Term)

Produktnummer ABIN7835875

Der Armenischer Hamster Monoklonal anti-CXCR3 Antikörper wird verwendet zum Nachweis von CXCR3 in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für CXCR3 Antikörper (N-Term) (ABIN7835875)

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser CXCR3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: CXCR3-173

Produktdetails anti-CXCR3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Armenian Hamster monoclonal antibody to CD183

Spezifität: Recognizes mouse CD183

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Mouse N-terminus of CXCR3

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-Armenian Hamster IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu CXCR3

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Andere Bezeichnung: CXCR3

Hintergrund: GPR9, C-X-C chemokine receptor type 3, CXC-R3, CXCR-3, CKR-L2, G protein-coupled receptor 9, Interferon-inducible protein 10 receptor, IP-10 receptor, CD183

Gen-ID: 12766