Syndecan 1 Antikörper (PE)

Produktnummer ABIN7835747

Der PE-konjugierte Maus Monoklonal anti-Syndecan 1 Antikörper (Klon DL-101) (ABIN7835747) detektiert spezifisch Syndecan 1 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Syndecan 1 Antikörper (PE) (ABIN7835747)

Target: Syndecan 1 (SDC1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Syndecan 1 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: DL-101

Produktdetails anti-Syndecan 1 Antikörper (PE)

Verwendungszweck: Mouse monoclonal antibody PE labled to CD138

Spezifität: Recognizes human CD138

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: A mixture of U266 and XG-1 human myeloma cell lines

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu Syndecan 1

Target: Syndecan 1 (SDC1)

Andere Bezeichnung: SDC1

Hintergrund: SDC, Syndecan-1, SYND1, CD138

Gen-ID: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism