IL7R Antikörper (Alexa Fluor 647)

Produktnummer ABIN7835713

Der Maus Monoklonal anti-IL7R Antikörper wird verwendet zum Nachweis von IL7R in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für IL7R Antikörper (Alexa Fluor 647) (ABIN7835713)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL7R Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: A019D5

Produktdetails anti-IL7R Antikörper (Alexa Fluor 647)

Verwendungszweck: Mouse monoclonal antibody AF647 labled to CD127

Spezifität: Recognizes human CD127

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Recombinant human CD127

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Andere Bezeichnung: IL7R

Hintergrund: Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, CD127

Gen-ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size