IL2 Receptor beta Antikörper

Produktnummer ABIN7835697

Dieser Ratte Monoklonal Antikörper detektiert spezifisch IL2 Receptor beta in WB, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für IL2 Receptor beta Antikörper (ABIN7835697)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IL2 Receptor beta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: TM-beta1

Produktdetails anti-IL2 Receptor beta Antikörper

Verwendungszweck: Rat monoclonal antibody to CD122

Spezifität: Recognizes mouse CD122

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Rat T cell line expressing mouse CD122 ( IL-2Rbeta)

Anwendungsinformationen

Applikationshinweise: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Andere Bezeichnung: IL2RB

Hintergrund: Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, p75, CD122

Gen-ID: 16185

Pathways: JAK-STAT Signalweg, Growth Factor Binding